Biolegend, 100627, Brilliant Violet 510™ anti-mouse CD5 Antibody, 50μg

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations: Moudra A, et al. 2021. J Immunol. 206:2109. PubMed Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed Schropp V, et al. 2019. J Neuroinflammation. 16:111. PubMed Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed Louise V Webb et al. 2019. Immunity. 50(2):348-361 . PubMed
RRID: AB_2563930 (BioLegend Cat. No. 100627)
Structure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution: Thymocytes, T cells, B-1 cells
Function: Negative regulator of T-B cell interaction
Ligand/Receptor: CD72, gp35-37
Cell Type: B cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.
Gene ID: 12507
UniProt: View information about CD5 on UniProt.org
Clone: 53-7.3
Regulatory Status: RUO
Other Names: Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype: Rat IgG2a, κ