Biolegend, 102404, Biotin anti-mouse CD31 Antibody, 500μg

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).
Application References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations: Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed Tarban N, et al. 2022. Cells. 11:. PubMed Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed Xie T, et al. 2021. Sci Adv. 7: . PubMed Verena Sigl et al. 2017. Cell research. 26(7):761-74 . PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Hagan AS, et al. 2020. Development. 147:00:00. PubMed Aneas I, et al. 2021. Nat Commun. 12:6115. PubMed Wagh A, et al. 2013. Nanomedicine. 9:449. PubMed Elsarraj H, et al. 2013. J Cell Sci. 126:2446. PubMed Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Gu VW, et al. 2021. Stem Cell Reports. 16:106. PubMed Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed Budai Z, et al. 2021. Cells. 10:. PubMed De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed Kopinke D et al. 2017. Cell. 170(2):340-351 . PubMed Liu X, et al. 2021. iScience. 24(6):102551. PubMed Seong J, et al. 2018. Development. 145:14. PubMed
RRID: AB_312899 (BioLegend Cat. No. 102404)
Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, αV/β3 integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.
Gene ID: 18613
UniProt: View information about CD31 on UniProt.org
Clone: 390
Regulatory Status: RUO
Other Names: PECAM-1, EndoCAM
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.