Biolegend, 102802, Purified anti-mouse CD40 Antibody, 500μg

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 + hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedCostim, Block, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 1C10 antibody has been reported to stimulate B cell proliferation and block CD40-CD40L interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking5,6 of CD40 ligand binding, and in vitro stimulation of B cell proliferation1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102810). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102812) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Ferlin WG, et al. 1998. Eur. J. Immunol. 28:525. (FC, Costim) Ferlin WG, et al. 1996. Eur. J. Immunol. 26:2911. (Costim) Heath A, et al. 1994. Eur. J. Immunol. 24:1828. (Costim) Ashman RF, et al. 2005. Int. Immunol. 17:411. Pelßez B, et al. 2001. J. Immunol. 166:6608. (Block) Zhang P, et al. 2007. J. Immunol. 178:474. (Block) Lawson BR, et al. 2007. J. Immunol. 178:5366. Ponomarev ED, et al. 2007. J.Immunol.178:39. PubMed
Product Citations: Chi H, et al. 2022. Cells. 11: . PubMed Obieglo K, et al. 2019. Eur J Immunol. 49:1226. PubMed Zhang P, et al. 2007. J Immunol. 178:474. PubMed Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed Zhang Y, et al. 2020. Front Immunol. 11:1012. PubMed Ashman R, et al. 2005. Int Immunol. 0.99375. PubMed Hoves S, et al. 2018. J Exp Med. 215:859. PubMed Le Q, Maizels N 2015. PLoS One. 11: 1005411. PubMed Patel J, et al. 2022. Int J Biol Sci. 18:2670. PubMed Sakaguchi T, et al. 2020. Cell Reports. 31(10):107755. PubMed Graf R et al. 2019. Cell reports. 26(5):1098-1103 . PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Rastelli J, et al. 2008. Blood. 111:1448. PubMed Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
RRID: AB_312935 (BioLegend Cat. No. 102802)
Structure: TNFR superfamily, 48 kD
Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors
Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand/Receptor: CD40L (CD154)
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881. 3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
Gene ID: 21939
UniProt: View information about CD40 on UniProt.org
Clone: 1C10
Regulatory Status: RUO
Other Names: Bp50, TNFRSF5
Isotype: Rat IgG2a, κ