Biolegend, 103313, PerCP/Cyanine5.5 anti-mouse CD45RB Antibody, 25μg

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4 + T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Cloned mouse Th2 cell lines
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
Application References(PubMed link indicates BioLegend citation): Bottomly K, et al. 1989. Eur. J. Immunol. 19:617. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations: Giampaolo S, et al. 2023. iScience. 26:106234. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
RRID: AB_1953290 (BioLegend Cat. No. 103313) AB_2284707 (BioLegend Cat. No. 103314)
Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells
Function: Phosphatase, T cell development and activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, Thy1
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264
UniProt: View information about CD45RB on UniProt.org
Clone: C363-16A
Regulatory Status: RUO
Other Names: CD45RB
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.