Biolegend, 103703, Biotin anti-mouse CD49d Antibody, 50μg

CD49d is a 150 kD glycoprotein, also known as α 4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α 4 β 1 ) or LPAM-1 (integrin α 4 β 7 ) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The 9C10(MFR4.B) antibody, in combination with the R1-2 monoclonal antibody, completely blocks VCAM-1 binding to VLA-4.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: (C57BL/6 x A/J)F1 mouse fetal liver mas
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3 of acetone-fixed frozen sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103709 & 103710).
Application References(PubMed link indicates BioLegend citation): Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Jia W, et al. 2005. Blood 106:3854. (FC)
Product Citations: Sandow JJ, et al. 2021. Proteomics. 21:e2000244. PubMed
RRID: AB_313044 (BioLegend Cat. No. 103703)
Structure: Integrin family, associates with integrin β1 or β7, 150 kD
Distribution: T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function: T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor: VCAM-1, MAdCAM-1, fibronectin
Cell Type: B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722. 3. Berlin C, et al. 1993. Cell 74:185. 4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
Gene ID: 16401
UniProt: View information about CD49d on UniProt.org
Clone: 9C10 (MFR4.B)
Regulatory Status: RUO
Other Names: α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.