Biolegend, 104714, APC anti-mouse CD80 Antibody, 100μg

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
100μg
Verified Reactivity: Mouse
Reported Reactivity: Dog
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: CHO cell line transfected with mouse B7 (CD80)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).
Application References(PubMed link indicates BioLegend citation): Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC) Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP) Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) Ma XT, et al. 2006. Cancer Res. 66:1169. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. Turnquist HR, et al. 2007. J. Immunol. 178:7018. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations: An S, et al. 2022. Int J Mol Sci. 23:. PubMed Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed Yu J, et al. 2022. Nat Commun. 13:7903. PubMed Ding D, et al. 2023. J Med Chem. 66:1467. PubMed Yao RQ, et al. 2022. Theranostics. 12:4606. PubMed Miao L, et al. 2020. Theranostics. 0.7625. PubMed Català C, et al. 2022. iScience. 25:105078. PubMed Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed Diao L, et al. 2022. iScience. 25:105511. PubMed Onodera T, et al. 2012. Proc Natl Acad Sci U S A. 109:2485. PubMed Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed Škrnjug I, et al. 2014. PLoS One. 9:110150. PubMed Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed Ma J, et al. 2021. Curr Protoc. 1:e144. PubMed Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed Xue RY, et al. 2019. Front Immunol. 10:1372. PubMed Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed Mao L, et al. 2022. Front Immunol. 13:833418. PubMed Chou S, et al. 2016. J Control Release. 244:14-23. PubMed Assi H, et al. 2014. PLoS One. 9:96318. PubMed Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed Guan X, et al. 2022. Nat Commun. 13:2834. PubMed Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed Wang X, et al. 2022. J Biol Chem. 298:101561. PubMed Yi H, et al. 2021. Front Immunol. 12:719189. PubMed Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed Zhang J, et al. 2022. Biomater Res. 26:44. PubMed Peng KT, et al. 2019. Int J Nanomedicine. 14:469. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Knox T, et al. 2019. Sci Rep. 9:6136. PubMed Xu Y, et al. 2021. iScience. 24:103445. PubMed Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed Mino T, et al. 2019. Nucleic Acids Res. 47:8838. PubMed Angulo G, et al. 2021. eLife. 10:00. PubMed Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed Guo S, et al. 2021. Chronic Dis Transl Med. 7:254. PubMed
RRID: AB_313134 (BioLegend Cat. No. 104713) AB_313135 (BioLegend Cat. No. 104714)
Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
Gene ID: 12519
UniProt: View information about CD80 on UniProt.org
Clone: 16-10A1
Regulatory Status: RUO
Other Names: B7-1, B7, Ly-53
Isotype: Armenian Hamster IgG