Biolegend, 105301, Purified anti-mouse CD90.2 (Thy1.2) Antibody, 50μg

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca 2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, Costim, Depletion - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).
Application References(PubMed link indicates BioLegend citation): Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) Seaman WE. 1983. J. Immunol. 130:1713. (Deplete) Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim) Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC) Hardy B, et al. 2005. Int. Immunol. 17:615. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations: Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed Chemnitz J, et al. 2019. Sci Rep. 3.745138889. PubMed Cao Q, et al. 2018. J Am Soc Nephrol. 29:961. PubMed Aguilar EG, et al. 2021. Blood Adv. 5:4219. PubMed Wang Y, et al. 2021. Cell Rep. 37:109695. PubMed Moskwa N, et al. 2022. Development. 149:. PubMed Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Zhang S, et al. 2020. Nat Commun. 11:2027. PubMed Yang W, et al. 2021. Hepatology. . PubMed Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed Yoon S, et al. 2020. Antioxidants (Basel). 10:. PubMed Sungur C, et al. 2013. Proc Natl Acad Sci U S A. 110:7401. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed Nishio T, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID: AB_313172 (BioLegend Cat. No. 105301) AB_313173 (BioLegend Cat. No. 105302)
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410.
Gene ID: 21838
UniProt: View information about CD90.2 on UniProt.org
Clone: 30-H12
Regulatory Status: RUO
Other Names: Thy-1.2
Isotype: Rat IgG2b, κ