Biolegend, 105606, FITC anti-mouse CD102 Antibody, 500μg

CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.

Product Details
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: COS cells transfected with mouse ICAM-2 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 105608).
Application References(PubMed link indicates BioLegend citation): Xu H, et al. 1996. J. Immunol. 156:4909.Gerwin N, et al. 1999. Immunity 10:9.
Product Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed Guilliams M, et al. 2022. Cell. 185:379. PubMed Wang YT, et al. 2020. Nat Microbiol. 0.397222222. PubMed Joffre J, et al. 2021. Crit Care Med. 49:e315. PubMed
RRID: AB_313199 (BioLegend Cat. No. 105606)
Structure: Ig superfamily, 55 kD
Distribution: Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
Function: Lymphocyte recirculation, costimulation
Ligand/Receptor: LFA-1, Mac-1
Cell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017. 3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
Gene ID: 15896
UniProt: View information about CD102 on UniProt.org
Clone: 3C4 (MIC2/4)
Regulatory Status: RUO
Other Names: ICAM-2
Isotype: Rat IgG2a, κ