Biolegend, 105813, PE/Cyanine7 anti-mouse CD117 (c-Kit) Antibody, 25μg

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bone marrow mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
Application References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed Ray S, et al. 2022. Elife. 11:. PubMed Han X, et al. 2023. J Clin Invest. 133:. PubMed Siatra P, et al. 2023. NPJ Regen Med. 8:13. PubMed Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed Zhao D, et al. 2021. Innate Immun. 27:533. PubMed Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed Van Deren DA, et al. 2022. Development. 149: . PubMed McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed García-García A, et al. 2023. Bioact Mater. 24:174. PubMed Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed Shiozawa Y, et al. 2010. PLoS One. 5:e10853. PubMed Ray S, et al. 2020. J Biol Chem. 295:7113. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Minutti CM, et al. 2019. Immunity. 50:645. PubMed Turner JA, et al. 2020. Immunity. 53:1202. PubMed Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed Chen X, et al. 2021. Theranostics. 11:4655. PubMed Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed Dráberová L, et al. 2020. Front Immunol. 10:3068. PubMed Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed Nakano N, et al. 2008. J Allergy Clin Immunol. 123:74. PubMed Simon M, et al. 2019. Cell Metab. 29:871. PubMed De S, et al. 2018. Development. 145. PubMed Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed Chen I, et al. 2016. Sci Rep. 6:27195. PubMed Obata-Ninomiya K, et al. 2013. J Exp Med. 210:2583. PubMed McAlpine CS, et al. 2021. Nature. 595:701. PubMed Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed Nakajima T, et al. 2021. J Exp Med. 218:. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed Shen YC, et al. 2021. Nat Commun. 12:3876. PubMed Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed Shibata F, et al. 2009. Stem Cells. 27:183. PubMed Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed Chen Z, et al. 2019. J Exp Med. 216:152. PubMed Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed Mehta C, et al. 2021. Nucleic Acids Res. 49:9007. PubMed Wei Q, et al. 2020. Dev Cell. 53:503. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed Joseph J, et al. 2012. Mol Cancer Res. 0.681944444. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed Sulsenti R, et al. 2021. Frontiers in Immunology. 12:622001. PubMed Xu H, et al. 2012. Blood. 120:3089. PubMed Tung LT, et al. 2021. Cell Death Dis. 12:923. PubMed Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed Mesa-Nuñez C, et al. 2022. Mol Ther Methods Clin Dev. 26:459. PubMed Wilkinson AC, et al. 2019. Nature. 571:117. PubMed Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed Gawish R, et al. 2022. Elife. 11:. PubMed
RRID: AB_313222 (BioLegend Cat. No. 105813) AB_313223 (BioLegend Cat. No. 105814)
Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Stem Cell Factor (SCF)
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.
Gene ID: 16590
UniProt: View information about CD117 on UniProt.org
Clone: 2B8
Regulatory Status: RUO
Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype: Rat IgG2b, κ