Biolegend, 106123, PE/Cyanine5 anti-mouse CD137 Antibody, 25μg

CD137 is a 39 kD TNF-receptor superfamily member also known as 4-1BB, TNFRSF9, Ly-63, and ILA (Induced by Lymphocyte Activation). CD137 is expressed as monomers, dimers, and tetramers on activated T and B cells. Interaction of CD137 with its ligand, 4-1BBL (CD137L), is important in T-B cell costimulation. The 17B5 antibody has been reported to block 4-1BBL costimulated T cell proliferation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106108).
Application References(PubMed link indicates BioLegend citation): Lee SW, et al. 2005. J. Immunol. 174:6803. (FC) Zheng G, et al. 2004. J. Immunol. 173:2428. (Block) Zheng H and G. G. Meadows 2005. J. Leukocyte Biol. 78:1070. (FC) PubMed Nishimoto H, et al. 2005. Blood 106:4241. (FC) del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
RRID: AB_3674967 (BioLegend Cat. No. 106123) AB_3674967 (BioLegend Cat. No. 106124)
Structure: TNF-R superfamily, 39 kD
Distribution: Activated T cells and B cells
Function: T-B cell costimulation
Ligand/Receptor: 4-1BB ligand (CDw137L)
Cell Type: B cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219. 3. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 21942
UniProt: View information about CD137 on UniProt.org
Clone: 17B5
Regulatory Status: RUO
Other Names: 4-1BB, TNFRSF9, Ly-63, ILA (Induced by Lymphocyte Activation), CD137
Isotype: Syrian Hamster IgG