Biolegend, 107213, PE/Cyanine7 anti-mouse CD273 (B7-DC, PD-L2) Antibody, 25μg

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse B7-DC transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1,6, blocking2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107221-107226).
Application References(PubMed link indicates BioLegend citation): Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB) Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block) Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC) Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block) Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block) Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations: MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Dichtl S, et al. 2021. Sci Adv. 7: . PubMed Cervera–Carrascon V, et al. 2020. Oncoimmunology. 9:1761229. PubMed Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
RRID: AB_2728122 (BioLegend Cat. No. 107213) AB_2728123 (BioLegend Cat. No. 107214)
Structure: B7 Immunoglobulin superfamily, 42 kD
Distribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues
Function: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor: PD-1, uncharacterized receptor
Cell Type: Dendritic cells, Macrophages
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Shin T, et al. 2003. J. Exp. Med. 198:31. 2. Liu X, et al. 2003. J. Exp. Med. 197:1721. 3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
Gene ID: 58205
UniProt: View information about CD273 on UniProt.org
Clone: TY25
Regulatory Status: RUO
Other Names: PD-L2, PDL2, B7DC, CD273
Isotype: Rat IgG2a, κ