Biolegend, 107803, PE anti-mouse CD321 (F11R) Antibody, 25μg

F11R is a cell adhesion molecule and immunoglobulin superfamily member and an important regulator of tight junction assembly in epithelia. It is found on the surface of platelets, and plays a role in platelet aggregation, secretion, adhesion, and spreading. By interacting with integrin alpha-L/beta-2, it is involved in leukocyte transmigration.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E14.5 mouse fetal liver cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Sugano Y et al. 2008. Blood. 111(3): 1167–72.
RRID: AB_2910281 (BioLegend Cat. No. 107803) AB_2910281 (BioLegend Cat. No. 107804)
Structure: 32 kD single pass type I membrane glycoprotein, member of the immunoglobin superfamily
Distribution: Cell membrane, tight junctions
Function: Protein homodimerization, integrin binding, PDZ domain binding, tight junction formation
Interaction: integrin alpha-L/beta-2, MPDZ, PARD3, ITGAL
Ligand/Receptor: integrin alpha-L/beta-2
Cell Type: Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Martin-Padura I, et al. 1998. J Cell Biol. 142:117-27. Muller WA. 2003. Trends Immunol. 24:327-34. Kostrewa D, et al. 2001. EMBO J. 20:4391-8.
Gene ID: O88792
UniProt: View information about CD321 on UniProt.org
Clone: 27-9
Regulatory Status: RUO
Other Names: Junctional adhesion molecule-1, Jam-1, Jam-A, junctional adhesion molecule-A, CD321
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.