Biolegend, 108123, PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1) Antibody, 25μg

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-dependent mouse T-cell line (CTL-L)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9. The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14
Application References(PubMed link indicates BioLegend citation): Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP) Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB) Codias EK, et al. 1990. J. Immunol. 144:2197. Malek TR, et al. 1986. J. Exp. Med. 164:709. Codias EK, et al. 1990. J. Immunol. 145:1407. Ivanov V, et al. 1994. J. Immunol. 153:2394. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. Fleming T, et al. 1994. J. Immunol. 153:1955. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC) Umland O, et al. 2007. J. Immunol. 178:4147. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed Pronk CJ, et al. 2011. J. Exp Med. PubMed English A, et al. 2000. J. Immunol. 165:3763. (IHC) Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations: Tober J, et al. 2020. The Journal of Experimental Medicine. 215(2):645-659. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed Helm M, et al. 2022. Life (Basel). 12:. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Taube C, et al. 2011. PLoS One. 6:e21799. PubMed Barman PK, et al. 2019. J Immunol. 202:2720. PubMed Reddy A, et al. 2020. Cell. 183:62. PubMed Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed Bajaña S, et al. 2022. iScience. 25:103732. PubMed van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed Gago-Lopez N, et al. 2019. EMBO Mol Med. 11:e10697. PubMed Pascual R, et al. 2020. Sci Adv. 6:eaax3868. PubMed Li K, et al. 2020. Nat Commun. 0.795138889. PubMed Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID: AB_893619 (BioLegend Cat. No. 108123) AB_893615 (BioLegend Cat. No. 108124)
Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Cell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.
Gene ID: 110454
UniProt: View information about Ly-6A/E on UniProt.org
Clone: D7
Regulatory Status: RUO
Other Names: Sca-1
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.