Biolegend, 108316, PerCP/Cyanine5.5 anti-mouse Ly-51 Antibody, 100μg

Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP) Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP) Adkins B, et al. 1988. Immunogenetics 27:180. (IHC) Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC) Goverman J, et al. 1997. Immunol. Today 18:204. (IHC) Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC) Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
Product Citations: Xing W, et al. 2024. J Clin Invest. :134. PubMed Chen Y, et al. 2022. Stem Cells Int. 2022:1309684. PubMed
RRID: AB_2632657 (BioLegend Cat. No. 108315) AB_2632658 (BioLegend Cat. No. 108316)
Structure: Aminopeptidase A, 140 kD
Distribution: B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells
Function: Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
Cell Type: B cells, Dendritic cells, Epithelial cells
Biology Area: Immunology
Antigen References: 1. Goverman J, et al. 1997. Immunol. Today 18:204. 2. Morse HC, et al. 1987. J. Exp. Med. 165:920. 3. Sherwood P, et al. 1990. Int. Immunol. 2:399. 4. Lin Q, et al. 1998. J. Immunol. 160:4681.
Gene ID: 13809
UniProt: View information about Ly-51 on UniProt.org
Clone: 6C3
Regulatory Status: RUO
Other Names: 6C3/BP-1
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.