Biolegend, 108904, Biotin anti-mouse CD49b (pan-NK cells) Antibody, 500μg

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α 2 integrin, VLA-2 α chain, and integrin α 2 chain. CD49b non-covalently associates with CD29 (β 1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-propagated NK1.1+ cells from C57BL/6 mice
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.
Application References(PubMed link indicates BioLegend citation): Arase H, et al. 2001. J. Immunol. 167:1141. (FC) Sepulveda H, et al. 1999. J. Immunol. 163:1133. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC) Andoniou CE, et al. 2005. Nature Immunology 6:1011. Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed Busche A, et al. 2011. J. Immunol. 186:2918. PubMed Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed Honjo K, et al. 2012. PNAS. PubMed. Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed
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RRID: AB_313410 (BioLegend Cat. No. 108903) AB_313411 (BioLegend Cat. No. 108904)
Structure: Integrin α chain, 150 kD
Distribution: NK cells, subset of T cells
Function: Adhesion
Ligand/Receptor: Collagen, laminin
Cell Type: NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Arase H, et al. 2001. J. Immunol. 167:1141. 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769.
Gene ID: 16398
UniProt: View information about CD49b on UniProt.org
Clone: DX5
Regulatory Status: RUO
Other Names: α2 integrin, VLA-2 α chain, DX5, Integrin α2 chain, ITGA2
Isotype: Rat IgM, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.