Biolegend, 108917, Pacific Blue™ anti-mouse CD49b (pan-NK cells) Antibody, 25μg

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α 2 integrin, VLA-2 α chain, and integrin α 2 chain. CD49b non-covalently associates with CD29 (β 1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-propagated NK1.1+ cells from C57BL/6 mice
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.
Application References(PubMed link indicates BioLegend citation): Arase H, et al. 2001. J. Immunol. 167:1141. (FC) Sepulveda H, et al. 1999. J. Immunol. 163:1133. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC) Andoniou CE, et al. 2005. Nature Immunology 6:1011. Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed Busche A, et al. 2011. J. Immunol. 186:2918. PubMed Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed Honjo K, et al. 2012. PNAS. PubMed. Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed
Product Citations: Quinn K, et al. 2013. J Immunol. 191:5085. PubMed Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed Schneider C, et al. 2018. Cell. 174:271. PubMed Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed Deady L, et al. 2014. Infect Immun . 82:1982. PubMed Flommersfeld S, et al. 2021. Immunity. :. PubMed Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Yu X, et al. 2016. Blood. 127: 132 - 138. PubMed Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
RRID: AB_2249376 (BioLegend Cat. No. 108917) AB_2265144 (BioLegend Cat. No. 108918)
Structure: Integrin α chain, 150 kD
Distribution: NK cells, subset of T cells
Function: Adhesion
Ligand/Receptor: Collagen, laminin
Cell Type: NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Arase H, et al. 2001. J. Immunol. 167:1141. 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769.
Gene ID: 16398
UniProt: View information about CD49b on UniProt.org
Clone: DX5
Regulatory Status: RUO
Other Names: α2 integrin, VLA-2 α chain, DX5, Integrin α2 chain, ITGA2
Isotype: Rat IgM, κ