Biolegend, 109815, Alexa Fluor® 488 anti-mouse CD45.2 Antibody, 25μg

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: B10.S mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
Application References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations: Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed Özcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed Peterson EJ, et al. 2019. Mol Syst Biol. 15:e8584. PubMed Li X, et al. 2023. J Clin Invest. 133:. PubMed Read KA, et al. 2023. Nat Commun. 14:1652. PubMed Florian DC, et al. 2023. Cancer Res Commun. 3:223. PubMed Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Wen T, et al. 2013. Proc Natl Acad Sci U S A. 110:6067. PubMed Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed Wang L, et al. 2020. Cells. 0.458333333. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed Tang Q, et al. 2021. Immunity. 54:1961. PubMed Akkaya M, et al. 2020. Sci Rep. 10:13630. PubMed Wang X, et al. 2021. EMBO J. 40:e105926. PubMed Hirasawa M, et al. 2016. J Biol Chem. 291: 7373-7385. PubMed Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed Zhang F, et al. 2019. Front Immunol. 2.077777778. PubMed Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
RRID: AB_492869 (BioLegend Cat. No. 109815) AB_492868 (BioLegend Cat. No. 109816)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.
Gene ID: 19264
UniProt: View information about CD45.2 on UniProt.org
Clone: 104
Regulatory Status: RUO
Other Names: Ly-5.2, LCA
Isotype: Mouse (SJL) IgG2a, κ