Biolegend, 109906, FITC anti-mouse I-A, 500μg

The 10-3.6 antibody reacts with the β chain of the I-A k MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-A f,r,s antigens and I-A g7 of NOD mice; it does not react with other haplotypes ( e.g., b, d, p, q).
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections. This clone does not cross react with other haplotypes (e.g. b, d, p, q).
Application References(PubMed link indicates BioLegend citation): Landais D, et al. 1986. J. Immunol. 137:3002. (IP) Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP) Kappler JW, et al. 1981. J. Exp. Med. 153:1198. Alisauskas RM, et al. 1986. Immunopharmacology 12:1. Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC) Yui MA, et al. 2010. J. Immunol. 185:284. PubMed Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
Product Citations: Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed Bi CS, et al. 2020. Cell Prolif. 53:e12827. PubMed Lai JH, et al. 2021. iScience. 24(6):102498. PubMed Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
RRID: AB_313454 (BioLegend Cat. No. 109905)
Structure: β chain of MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14961
UniProt: View information about I-Ak on UniProt.org
Clone: 10-3.6
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Mouse (CWB) IgG2a, κ