Biolegend, 109913, Brilliant Violet 421™ anti-mouse I-A, 50μg

The 10-3.6 antibody reacts with the β chain of the I-A k MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-A f,r,s antigens and I-A g7 of NOD mice; it does not react with other haplotypes ( e.g., b, d, p, q).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections. This clone does not cross react with other haplotypes (e.g. b, d, p, q).
Application References(PubMed link indicates BioLegend citation): Landais D, et al. 1986. J. Immunol. 137:3002. (IP) Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP) Kappler JW, et al. 1981. J. Exp. Med. 153:1198. Alisauskas RM, et al. 1986. Immunopharmacology 12:1. Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC) Yui MA, et al. 2010. J. Immunol. 185:284. PubMed Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
RRID: AB_3662360 (BioLegend Cat. No. 109913)
Structure: β chain of MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14961
UniProt: View information about I-Ak on UniProt.org
Clone: 10-3.6
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Mouse (CWB) IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.