Biolegend, 110102, Purified anti-mouse CD27 Antibody, 100μg

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, CD27 is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD27 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A18209B can compete with clone LG.3A10 on target cells. Clone A18209B can also block binding of CD70 to CD27 in a cell-based binding assay.
RRID: AB_2910285 (BioLegend Cat. No. 110102)
Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocytes subset
Function: Costimulatory signal for T and B cell activation, T cell development
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor: CD70
Cell Type: B cells, Lymphocytes, T cells, Thymocytes
Biology Area: Adaptive Immunity, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Hintzen RQ, et al. 1994. Immunol. Today 15:307-11. Akiba H, et al. 1998. J. Biol. Chem. 273:13353-8. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249-53. Camerini D, et al. 1991. J. Immunol. 147:3165-9.
Gene ID: 21940
UniProt: View information about CD27 on UniProt.org
Clone: A18209B
Regulatory Status: RUO
Other Names: T14, S152, Tp55, TNFRSF7
Isotype: Rat IgG2a, κ