Biolegend, 110721, Pacific Blue™ anti-mouse CD45.1 Antibody, 25μg

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SJL mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).
Application References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations: Le DT, et al. 2023. iScience. 26:106059. PubMed Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed Cheng H, et al. 2023. Nat Metab. 5:314. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Page N, et al. 2018. Immunity. 48:937. PubMed Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Lin G, et al. 2013. J Immunol. 190:4627. PubMed Liu X, et al. 2021. eLife. 0.416666666666667. PubMed Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed Li L, et al. 2010. J Immunol. 184:1728. PubMed Müller M, et al. 2021. Nat Commun. 12:7036. PubMed Wigton E, et al. 2016. J Leukoc Biol. 100: 143 - 153. PubMed Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed Cheng Y, et al. 2021. Cancer Cell. . PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed Kalinski AL, et al. 2020. Elife. 9:00. PubMed Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed Chen X, et al. 2021. Cell Rep. 37:109991. PubMed Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Holian C 2007. Am J Respir Cell Mol Biol. 37:729. PubMed Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed Florez MA, et al. 2020. Cell Rep. 33:108530. PubMed Mills R, et al. 2015. J Immunol. 194:3675. PubMed Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed Fiege JK, et al. 2019. J Immunol. 203(4):936-945. PubMed Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed Diao J, et al. 2018. J Immunol. 201:1306. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_492867 (BioLegend Cat. No. 110721) AB_492866 (BioLegend Cat. No. 110722)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264
UniProt: View information about CD45.1 on UniProt.org
Clone: A20
Regulatory Status: RUO
Other Names: T200, Ly-5.1, LCA
Isotype: Mouse (A.SW) IgG2a, κ