Biolegend, 112003, Alexa Fluor® 488 anti-mouse CD146 Antibody, 25μg

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFN-γ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein (val 24-val 563 extracellular domain)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3068180 (BioLegend Cat. No. 112003) AB_3068180 (BioLegend Cat. No. 112004)
Structure: CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
Distribution: CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.
Function: CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
Ligand/Receptor: Unidentified ligand
Cell Type: Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Gene ID: 84004
UniProt: View information about CD146 on UniProt.org
Clone: A20078A
Regulatory Status: RUO
Other Names: S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
Isotype: Rat IgG2b, κ