Biolegend, 112206, Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) Antibody, 100μg

Ep-CAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of Ep-CAM seems to correlate inversely with the level of E-cadherin (CD324). Ep-CAM is considered important in tumor biology.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
RRID: AB_3683140 (BioLegend Cat. No. 112205) AB_3683140 (BioLegend Cat. No. 112206)
Structure: 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution: Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types
Function: Mediates calcium-independent homophilic cell-cell adhesion
Interaction: CD326 displays hemophilic binding.
Ligand/Receptor: CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Antigen References: Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4. Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
Gene ID: 17075
UniProt: View information about CD326 on UniProt.org
Clone: W20119E
Regulatory Status: RUO
Other Names: EGP40, MIC18, TROP1, KSA
Isotype: Rat IgG2b, κ