Biolegend, 114003, PE anti-mouse CCRL2 Antibody, 25μg

CCRL2 (C-C chemokine receptor-like 2) is also known as Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR/Lccr). CCRL2 is a chemokine receptor-like, 7-transmembrane protein that can bind CCL19 or chemerin/RARRES2. Mouse CCRL2 is expressed by several leukocytes, including dendritic cells, macrophages, neutrophils, and microglia and is rapidly upregulated following stimulation with proinflammatory stimuli, such as LPS and TNF-α. CCRL2 plays various roles in regulating chemokine activities, e.g., by binding and internalizing excess local levels of the CCL19/MIP-3β chemokine. Alternatively, endothelial cell-expressed CCRL2 can bind & present the chemerin/RARRES2 chemoattractant to lymphoid cells that express the functional signaling receptor, CMKLR1, and promote their adhesion.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCRL2-Transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S21013E completely blocks the reference clone BZ2E3.
RRID: AB_3068196 (BioLegend Cat. No. 114003) AB_3068196 (BioLegend Cat. No. 114004)
Structure: C-C chemokine receptor-like 2 (CCRL2) is a non-signaling seventransmembrane domain (7-TMD) receptor related to the atypical chemokine receptor (ACKR) family.
Distribution: Expressed in many leukocytes, including dendritic cells, macrophages, neutrophils, mast cells, microglia & astrocytes
Function: CC-chemokine receptor-like 2 (CCRL2) binds leukocyte chemoattractant chemerin and can regulate local levels of the attractant, but does not itself support cell migration.
Interaction: Macrophages, Dendritic Cells, Mast Cells
Ligand/Receptor: CCL19 and chemerin/RARRES2 bind to CCRL2
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Monnier J, et al. 2012. J Immunol. 2012; 189:956-67. Shimada T, et al. 1998. FEBS Lett. 1998; 425:490-4 Yoshimura T, et al. 2011. Exp Cell Res. 317:674-84. Zabel BA, et al. 2008. J Exp Med. 205:2207-20
Gene ID: 54199
UniProt: View information about CCRL2 on UniProt.org
Clone: S21013E
Regulatory Status: RUO
Other Names: C-C chemokine receptor-like 2, L-CCR, Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR), Cmkbr1l2, Ackr5
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.