Biolegend, 114604, Biotin anti-mouse H-2K, 500μg

The 28-8-6 antibody reacts with the H-2K b and H-2D b MHC class I alloantigens expressed on nucleated cells from mice of the H-2K b /H-2D b haplotype. H-2K b /H-2D b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2D d MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt) Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC) Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
Product Citations: Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed Bui J, et al. 2006. J Immunol. 176:905. PubMed
RRID: AB_313594 (BioLegend Cat. No. 114603)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb/H-2Db haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Ozato K, et al. 1981. J. Immunol. 126:317. 2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447. 3. Evans GA, et al. 1992. Nature 300:755.
Gene ID: 1496414972
UniProt: View information about H-2Kb/H-2Db on UniProt.org
Clone: 28-8-6
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (C3H) IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.