Biolegend, 114605, FITC anti-mouse H-2K, 50μg

The 28-8-6 antibody reacts with the H-2K b and H-2D b MHC class I alloantigens expressed on nucleated cells from mice of the H-2K b /H-2D b haplotype. H-2K b /H-2D b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2D d MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt) Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC) Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
Product Citations: Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed Bruna A, et al. 2016. Cell. 167: 260-274. PubMed Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed Liu X, et al. 2020. Nature. . PubMed Lu SX, et al. 2021. Cell. . PubMed He X, et al. 2009. Stem Cells. 27:1487. PubMed Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed Shen M, et al. 2022. Nat Cancer. 3:60. PubMed Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed Saveljeva S, et al. 2022. Cell Metab. 34:106. PubMed Kang YH, et al. 2019. Nat Commun. 10:912. PubMed Ueda N, et al. 2006. Int Immunol. 1.720138889. PubMed Ng S, et al. 2022. Sci Rep. 12:14087. PubMed Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed Tran NL, et al. 2022. Elife. 11:. PubMed Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed Bui J, et al. 2006. J Immunol. 176:905. PubMed
RRID: AB_313596 (BioLegend Cat. No. 114605) AB_313597 (BioLegend Cat. No. 114606)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb/H-2Db haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Ozato K, et al. 1981. J. Immunol. 126:317. 2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447. 3. Evans GA, et al. 1992. Nature 300:755.
Gene ID: 1496414972
UniProt: View information about H-2Kb/H-2Db on UniProt.org
Clone: 28-8-6
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (C3H) IgG2a, κ