Biolegend, 114706, FITC anti-mouse H-2K, 500μg

The 34-1-2S antibody reacts with the H-2K d /H-2D d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K d /H-2D d haplotype. H-2K d /H-2D d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1982. Transplantation 34:113. Sester M, et al. 2000. J. Biol. Chem. 34:113.
Product Citations: Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Chen Y, et al. 2022. Am J Cancer Res. 12:5286. PubMed Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed Li X, et al. 2022. Nat Commun. 13:2794. PubMed Liu X, et al. 2020. Nature. . PubMed Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed Tassi I, et al. 2014. J Immunol. 193:4303. PubMed Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
RRID: AB_313605 (BioLegend Cat. No. 114706)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kd/H-2Dd haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 1496414972
UniProt: View information about H-2Kd/H-2Dd on UniProt.org
Clone: 34-1-2S
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ