Biolegend, 115505, FITC anti-mouse CD19 Antibody, 50μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed Tan J, et al. 2021. iScience. 24(8):102835. PubMed Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Furuhashi K, et al. 2017. Immunology. 151:227. PubMed Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed Tang C, et al. 2023. Nat Commun. 14:1493. PubMed Tsugawa H, et al. 2023. PLoS Pathog. 19:e1011139. PubMed Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed Collin R, et al. 2020. J Immunol. 205:133. PubMed Li X, et al. 2022. J Clin Invest. 132: . PubMed Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed He Y, et al. 2022. Front Immunol. 13:986202. PubMed Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed Hattori K, et al. 2023. Biomed Rep. 18:1. PubMed Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Chen CC, et al. 2021. J Exp Med. 218:. PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Turner JA, et al. 2020. Immunity. 53:1202. PubMed Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed Forte E, et al. 2020. J Cell Mol Med. . PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed Das S, et al. 2019. Oncogene. 383316. PubMed Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed Hoffman D, et al. 2021. Immunity. 54:2712. PubMed Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed Rothweiler S, et al. 2019. Purinergic Signal. 0.885416667. PubMed Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed Haase C, et al. 2022. Nat Methods. 19:1622. PubMed Ma J, et al. 2016. Proc Natl Acad Sci U S A. 113: 450 - 458. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Omenetti S, et al. 2015. Cell Mol Gastroenterol Hepatol. 1: 406-419. PubMed Burrack K, et al. 2015. J Immunol. 194:678. PubMed Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed Sun S, et al. 2013. J Immunol. 190:6209. PubMed Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed Tuong ZK, et al. 2021. iScience. 24:103326. PubMed Vacca F, et al. 2020. eLife. 9:e54017.. PubMed Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed Xu Y, et al. 2021. iScience. 24:103445. PubMed Harrison DK, et al. 2021. Transplantation. 105:540. PubMed Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed Wang X, et al. 2022. Elife. 11:. PubMed Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed Zhang Y, et al. 2017. J Invest Dermatol. 137:2532. PubMed Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed Lai NY, et al. 2020. Cell. 180:33:00. PubMed Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed Fan X, et al. 2014. PLoS One. 9:107638. PubMed Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID: AB_313640 (BioLegend Cat. No. 115505) AB_313641 (BioLegend Cat. No. 115506)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ