Biolegend, 115509, PE/Cyanine5 anti-mouse CD19 Antibody, 25μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Tan J, et al. 2021. iScience. 24(8):102835. PubMed Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed Marangoni F, et al. 2021. Cell. . PubMed Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed Mamedov MR, et al. 2018. Immunity. 48:350. PubMed Petrus P, et al. 2020. Cell Metabolism. 31(2):375-390.e11.. PubMed Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed Kwok T, et al. 2022. Front Aging. 3:838943. PubMed Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed Carlson PM, et al. 2021. J Immunol. 207:720. PubMed Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed Kissiov DU, et al. 2022. Elife. 11:. PubMed Lam KC, et al. 2021. Cell. 184:5338. PubMed Sánchez-del-Campo L, et al. 2021. J Exp Clin Cancer Res. 40:117. PubMed
RRID: AB_313644 (BioLegend Cat. No. 115509) AB_313645 (BioLegend Cat. No. 115510)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ