Biolegend, 115521, Alexa Fluor® 488 anti-mouse CD19 Antibody, 100μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Arora S, et al. 2023. PLoS One. 18:e0286211. PubMed Jang HJ, et al. 2022. Cancer Res. 82:2593. PubMed Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed Chen C, et al. 2019. Cell Rep. 29:4200. PubMed Loh Z, et al. 2020. PLOS Pathogens. 16(7):e1008651. PubMed Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed Collado J, et al. 2013. Eur J Immunol. 43:2273. PubMed Wang J, et al. 2011. Am J Pathol. 178:2168. PubMed Zhu K, et al. 2022. EMBO Rep. 23:e54499. PubMed Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed Seeley-Fallen M, et al. 2014. Proc Natl Acad Sci U S A. 111:9881. PubMed Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed Song W, et al. 2022. Immunity. 55:290. PubMed
RRID: AB_493339 (BioLegend Cat. No. 115524) AB_389307 (BioLegend Cat. No. 115521)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ