Biolegend, 115526, Pacific Blue™ anti-mouse CD19 Antibody, 25μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Quinn K, et al. 2013. J Immunol. 191:5085. PubMed Cautivo KM, et al. 2022. Immunity. 55:254. PubMed Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed Ostendorf BN, et al. 2022. Nature. 611:346. PubMed Georgiev GI, et al. 2022. Cell Chem Biol. 29:811. PubMed Schneider C, et al. 2018. Cell. 174:271. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed Dema B, et al. 2014. J Exp Med. 211:2159. PubMed Dow L, et al. 2014. PLoS One. 9:95236. PubMed Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed Haso W, et al. 2013. Blood. 121:1165. PubMed Lang V, et al. 2021. Elife. 10:. PubMed Lin J, et al. 2021. Nat Commun. 12:5321. PubMed Minguet S, et al. 2017. Nat Immunol. 18:1150. PubMed Chen RJ, et al. 2022. iScience. 25:105595. PubMed Han S, et al. 2016. Arthritis Res Ther. 0.975. PubMed Kajikawa A, et al. 2015. PLoS One. 10: e0141713. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed Ding Y, et al. 2013. J Immunol. 191:1614. PubMed O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed Zheng Y, et al. 2022. J Immunol. 208:501. PubMed Korin B, et al. 2020. Sleep. :43. PubMed Molgora M, et al. 2020. Cell. 182:886. PubMed Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed Fiala G, et al. 2015. J Exp Med. 212: 1693-1708. PubMed Pfeil J, et al. 2020. Front Immunol. 11:529035. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Chen Z, et al. 2020. J Immunol. 204:335. PubMed Call M, et al. 2009. J Immunol. 182:6342. PubMed He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
RRID: AB_493341 (BioLegend Cat. No. 115526) AB_439718 (BioLegend Cat. No. 115523)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ