Biolegend, 115532, PerCP anti-mouse CD19 Antibody, 100μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Quah B, Parish C 2012. J Immunol Methods. 379:1. PubMed Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed Miyai T, et al. 2014. Proc Natl Acad Sci U S A. 111:1780. PubMed Han J, et al. 2018. BMC Res Notes. 11:229. PubMed Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed Luo Z, et al. 2020. Front Immunol. 0.883333333. PubMed Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed Ylösmäki E, et al. 2021. Mol Ther Oncolytics. 459:20. PubMed Ylösmäki E, et al. 2021. J Immunother Cancer. 9:. PubMed Dutow P, et al. 2014. J Infect Dis. 209:1269. PubMed Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed Fusciello M, et al. 2022. Mol Ther Oncolytics. 25:137. PubMed Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed Jesus M, et al. 2013. J Vis Exp. 73: 50167. PubMed Bergquist M, et al. 2014. J Infect. 69:113. PubMed Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
RRID: AB_893278 (BioLegend Cat. No. 115531) AB_2072926 (BioLegend Cat. No. 115532)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.