Biolegend, 116501, Purified anti-mouse H-2K, 50μg

The AF6-88.5 antibody reacts with H-2K b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K b haplotype. H-2K b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections. Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).
Application References(PubMed link indicates BioLegend citation): Loken MR, et al. 1982. J. Immunol. Methods 50:R85. Wall KA, et al. 1983. J. Immunol. 131:1056. Andersson M, et al. 1998. J. Immunol. 161:6475. Shao H, et al. 2005. J. Immunol. 175:1851. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
Product Citations: Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed Cai S, et al. 2020. Sci Rep. 10:14249. PubMed Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed Robert S, et al. 2018. Bone Marrow Transplant. 53:807. PubMed Chen HW, et al. 2021. Biomedicines. 9:. PubMed Stefanski HE, et al. 2018. PLoS One. 13:e0198871. PubMed Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed Shao H, et al. 2005. J Immunol. 175:1851. PubMed Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
RRID: AB_313728 (BioLegend Cat. No. 116501) AB_313729 (BioLegend Cat. No. 116502)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972
UniProt: View information about H-2Kb on UniProt.org
Clone: AF6-88.5
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (BALB/c) IgG2a, κ