Biolegend, 116518, APC anti-mouse H-2K, 100μg

The AF6-88.5 antibody reacts with H-2K b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K b haplotype. H-2K b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections. Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).
Application References(PubMed link indicates BioLegend citation): Loken MR, et al. 1982. J. Immunol. Methods 50:R85. Wall KA, et al. 1983. J. Immunol. 131:1056. Andersson M, et al. 1998. J. Immunol. 161:6475. Shao H, et al. 2005. J. Immunol. 175:1851. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
Product Citations: Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Sethumadhavan S, et al. 2017. PLoS One. . 10.1371/journal.pone.0187314. PubMed Regev O, et al. 2022. Front Immunol. 13:849701. PubMed Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed Gruber T, et al. 2020. JCI Insight. 5:00. PubMed Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed Perry JSA, et al. 2018. Immunity. 48:923. PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Wang X, et al. 2011. Blood. 118:6426. PubMed Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed Canton J, et al. 2021. Nat Immunol. 22:140. PubMed Gurusamy D, et al. 2020. Cancer Cell. 37(6):818-833.e9. PubMed Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed Kalbasi A, et al. 2020. Sci Transl Med. :12. PubMed Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed Chen X, et al. 2021. Cell Rep. 37:109991. PubMed Fujimoto K, et al. 2022. Sci Rep. 12:18313. PubMed Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed Yoshida S, et al. 2022. PLoS One. 17:e0264317. PubMed Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed Gejman RS et al. 2018. eLife. 7 pii: e41090. PubMed
RRID: AB_10568693 (BioLegend Cat. No. 116517) AB_10564404 (BioLegend Cat. No. 116518)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972
UniProt: View information about H-2Kb on UniProt.org
Clone: AF6-88.5
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (BALB/c) IgG2a, κ