Biolegend, 117002, Purified anti-mouse CD106 Antibody, 100μg

CD106, also known as VCAM-1 and INCAM-110, is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Transfectant cells (aa25-692) and recombinant protein (aa25-692)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-P, IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Flow cytometry testing showed that clone W21127K does not affect the bindings of clones 429 (MVCAM.A) and W21127Q on target cells.
RRID: AB_3097228 (BioLegend Cat. No. 117002)
Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand/Receptor: VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Kinashi T, et al. 1995. J Leukoc Biol. 57:168-73. Bevilacquea MP. 1993. Annu Rev Immunol. 11:767-804. Koni PA, et al. 2001. J Exp Med. 193:741-54.
Gene ID: 22329
UniProt: View information about CD106 on UniProt.org
Clone: W21127K
Regulatory Status: RUO
Other Names: VCAM-1, INCAM-110
Isotype: Rat IgG2a, κ