Biolegend, 120222, PE/Cyanine7 anti-mouse CD357 (GITR) Antibody, 100μg

GITR, Glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD4 + CD25 + T regulatory (Treg) cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4 + CD25 + Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse GITR protein and Transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 120216).
Application References(PubMed link indicates BioLegend citation): Cobbold SP, et al. 2004. J. Immunol. 172:6003. Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed
Product Citations: Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed An J, et al. 2022. iScience. 25:103570. PubMed Yue X, et al. 2019. Nat Commun. 10:2011. PubMed Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
RRID: AB_528907 (BioLegend Cat. No. 120222)
Structure: TNF receptor superfamily member
Distribution: Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation.
Function: Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand/Receptor: GITR ligand (TNFSF18)
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059 2. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:613 3. Kanamaru F, et al. 2004. J. Immunol. 172:613 4. Shimizu J, et al. 2002. Nat. Immunol. 3:135 5. Stephens GL, et al. 2004. J. Immunol. 173:5008 6. McHugh RS, et al. 2002. Immunity 16:311
Gene ID: 21936
UniProt: View information about CD357 on UniProt.org
Clone: YGITR 765
Regulatory Status: RUO
Other Names: TNFRSF18, AITR
Isotype: Rat IgG2b, κ