Biolegend, 121421, Brilliant Violet 421™ anti-mouse CD103 Antibody, 125microl

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin α IEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β 7 , CD103 is expressed as αE/β 7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse intestinal intraepithelial lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.
Application References(PubMed link indicates BioLegend citation): LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP) Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF) Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations: Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Eschweiler S 2023. Cell Reports. 42(2):112040. PubMed Bilate AM, et al. 2020. Immunity. 53(5):1001-1014.e20. PubMed Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed Woyciechowski S, Hofmann M, Pircher H 2017. Eur J Immunol. 47:244-250. PubMed Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed Monaghan KL, et al. 2020. J Vis Exp. . PubMed Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed Highton AJ, et al. 2019. Wellcome Open Res. 4:78. PubMed Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
RRID: AB_10900074 (BioLegend Cat. No. 121421) AB_2562901 (BioLegend Cat. No. 121422)
Structure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.
Function: Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor: E-Cadherin
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.
Gene ID: 16407
UniProt: View information about CD103 on UniProt.org
Clone: 2E7
Regulatory Status: RUO
Other Names: Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype: Armenian Hamster IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.