Biolegend, 121614, APC anti-mouse CD107a (LAMP-1) Antibody, 100μg

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Product Citations: Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed Seliger C 2013. J Immunol. 190:3328. PubMed Okai B, et al. 2015. Infect Immun . 83:1523. PubMed Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed Chaurasiya S, et al. 2020. Oncoimmunology. 9:1729300. PubMed Bolinger B, et al. 2013. J Immunol. 190:4162. PubMed Livingston-Rosanoff D, et al. 2012. J Virol. 86:12879.. PubMed Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed Voigt EA, et al. 2022. NPJ Vaccines. 7:136. PubMed Sanmarco LM, et al. 2021. Nature. 590:473. PubMed Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed Ren Y, et al. 2021. Nat Commun. 12:5405. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Truckenbrod EN, et al. 2021. Elife. 10:. PubMed Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed Lyons DO, et al. 2018. PLoS One. 13:e0207704. PubMed Kujur W, et al. 2020. PLoS Pathog. 16:e1009132. PubMed Nabekura T, et al. 2020. Immunity. 96:52. PubMed Han J, et al. 2015. Cancer Res . 75: 5273 - 5282. PubMed
RRID: AB_2134486 (BioLegend Cat. No. 121613) AB_2234505 (BioLegend Cat. No. 121614)
Structure: LAMP-1 is a 406 amino acid protein with a molecular mass of 44 kD.
Gene ID: 16783
UniProt: View information about CD107a on UniProt.org
Clone: 1D4B
Regulatory Status: RUO
Other Names: Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1
Isotype: Rat IgG2a, κ