Biolegend, 121714, APC anti-mouse Qa-2 Antibody, 100μg

Mouse Qa-2 is a 40 kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of the H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than on B cells. Different mouse strains express Qa-2 at varying levels. Qa-2, in association with β2-microglobulin, binds to peptide and plays a role in antigen presentation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2.
Application References(PubMed link indicates BioLegend citation): Sharrow SO, et al. 1989. J. Immunol. 142:3495. Patk E, et al. 1993. Microbiol. Immunol. 37:743.
RRID: AB_2832435 (BioLegend Cat. No. 121713) AB_2832436 (BioLegend Cat. No. 121714)
Structure: GPI-linked protein, associates with β2-microglobulin, 40 kD
Distribution: T and B cells, mature T cells expression higher than B cells
Function: Antigen presentation, promote immune response
Ligand/Receptor: Peptide
Cell Type: B cells, T cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Sharrow SO, et al. 1989. J. Immunol. 142:3495. 2. Mann DW, et al. 1987. J. Immunol. 138:240. 3. Mellor AL, et al.1985. Proc. Natl. Acad. Sci.USA. 82:5920. 4. Tabaczewski P, et al. 1997. J. Immunol. 159:2771. 5. Chiang EY and Stroynowski I. 2005. J. Immunol. 174:5367.
Gene ID: 111458
UniProt: View information about Qa-2 on UniProt.org
Clone: 695H1-9-9
Regulatory Status: RUO
Other Names: Nonclassical MHC-Ib, H2-Qa2
Isotype: Mouse IgG2a, κ