Biolegend, 121910, Alexa Fluor® 647 anti-mouse CD309 (VEGFR2, Flk-1) Antibody, 100μg

The 89B3A5 antibody recognizes mouse CD309 also known as vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF or VEGFC; ligand binding plays a key role in vascular development and vascular permeability. The 89B3A5 antibody has been shown to be useful for flow cytometry.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Rat-1 cells transfected with full-length mouse Flk
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Kaburn N, et al. 1997. Development 124:2039.
Product Citations: Siatra P, et al. 2023. NPJ Regen Med. 8:13. PubMed Verhoeven J, et al. 2022. Methods Mol Biol. 2572:45. PubMed Rignault-Clerc S, et al. 2013. Burns. 39:694. PubMed Glaser D, et al. 2016. PLoS One. 11:e0166663. PubMed Tanaka T, et al. 2012. Blood. 120:1299. PubMed N. H, et al. 2017. Journal of Vascular Research. 10.1159/000464287. PubMed Moschetta M, et al. 2016. Leukemia. 10.1038/leu.2016.3. PubMed Rüder C, et al. 2014. PLoS One. 9:104644. PubMed Hamerlik P, et al. 2012. J Exp Med. 209:507. PubMed Wu Q, et al. 2013. Eur J Pharmacol. 707:87. PubMed Xie X, et al. 2018. FASEB J. 32:1296. PubMed
RRID: AB_756169 (BioLegend Cat. No. 121909) AB_756170 (BioLegend Cat. No. 121910)
Structure: Member of the tyrosine protein kinase family, sub-family CSF-1/PDGF. Single pass transmembrane receptor containing a protein kinase domain and seven immunoglobulin-like domains.
Distribution: Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels.
Function: Receptor for VEGF or VEGFC. Ligand binding plays a key role in vascular development and vascular permeability.
Ligand/Receptor: VEGF, VEGFC
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Patterson C, et al. 1995. J. Biol. Chem. 270:23111. 2. Quinn TP, et al. 1993. Proc. Natl. Acad. Sci. USA 90:7533.
Gene ID: 16542
UniProt: View information about CD309 on UniProt.org
Clone: 89B3A5
Regulatory Status: RUO
Other Names: Vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, fetal liver kinase-1
Isotype: Rat IgG2a, κ