Biolegend, 122203, APC anti-mouse IL-21 Antibody, 25μg

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine primarily produced by NKT cells and CD4 + T cells, particularly the inflammatory Th17 subset. It exerts pleiotropic effects on both innate and adaptive immune responses. These include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. IL-21 also has proapoptotic effects on B cells and NK cells. Recent studies have shown that IL-21 functions as an autocrine cytokine, strongly inducing Th17 differentiation and suppressing Foxp3 expression, making it a potential therapeutic target for treating cancer and inflammatory diseases.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_3683149 (BioLegend Cat. No. 122203) AB_3683149 (BioLegend Cat. No. 122204)
Structure: Interleukin
Distribution: CD4 activated T cells, Th17 cells, Th2 cells
Function: Immunomodulation
Ligand/Receptor: IL-21R
Cell Type: NKT cells, T cells, Tfh, Th17, Th2
Biology Area: Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Parrish-Novak J, et al. 2000. Nature. 408:57-63. Mehta DS, et el. 2004. Immunol Rev. 202:84-95.
Gene ID: 60505
UniProt: View information about IL-21 on UniProt.org
Clone: S20017B
Regulatory Status: RUO
Other Names: Interleukin-21
Isotype: Rat IgG1, κ