Biolegend, 123120, Alexa Fluor® 488 anti-mouse F4/80 Antibody, 100μg

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine macrophages
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F, 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg/ml. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.
Application References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Kretschmer I, et al. 2016. J Biol Chem. 291: 4091 - 4106. PubMed Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed Lu L, et al. 2023. Adv Sci (Weinh). 10:e2206212. PubMed Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed Graça FA, et al. 2023. Nat Commun. 14:2900. PubMed Zhang Z, et al. 2022. Front Pharmacol. 13:906625. PubMed Hutami IR, et al. 2022. Oral Dis. 28:1157. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed El-Naccache DW, et al. 2022. Cell Rep. 40:111150. PubMed Hao J, et al. 2022. Cell Rep. 41:111804. PubMed Leonardi I, et al. 2022. Cell. 185:831. PubMed Yan Q, et al. 2020. Commun Biol. 288:3. PubMed Dong Z, et al. 2013. PLoS One. 18:80364. PubMed Watanabe M et al. 2017. eLife. 6 pii: e26635. PubMed Bordignon P et al. 2019. Cell Rep. 28(9):2358-2372 . PubMed Tan L, et al. 2022. Biochem Biophys Rep. 32:101351. PubMed Zhang BZ, et al. 2022. JCI Insight. 7:. PubMed Carlock C, et al. 2013. Reproduction. 146:491. PubMed Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed Ogbechi J, et al. 2022. Front Immunol. 13:1001956. PubMed Bussi C, et al. 2022. Nat Commun. 13:7338. PubMed Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed Ohnishi A, et al. 2015. PLoS One. 10:117616. PubMed Galle E, et al. 2022. Genome Biol. 23:207. PubMed Brocq M, et al. 2014. J Immunol. 192:6120. PubMed Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed Niu S, et al. 2016. J Immunol. 197: 3293 - 3301. PubMed Tedford K, et al. 2017. Nat Commun. . 10.1038/s41467-017-02482-4. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed Linde N, et al. 2018. Nat Commun. 9:21. PubMed Bretscher P, et al. 2015. EMBO Mol Med. 7:593. PubMed Wheeler R, Gull E 2015. Nat Commun. 6: 8964. PubMed Wongchana W, et al. 2015. J Immunol. 195: 5337 - 5346. PubMed Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed Zhou Z, et al. 2022. Life (Basel). 12:. PubMed De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed Carlock C, et al. 2014. J Immunol. 193:161. PubMed De Simone G, et al. 2021. Immunity. :. PubMed He J, et al. 2019. J Clin Invest. 130. PubMed Li H, et al. 2019. Front Immunol. 2.205555556. PubMed Blriot C, et al. 2021. Immunity. :. PubMed Wang H, et al. 2016. PLoS One. 11:e0167307. PubMed Care A, et al. 2014. Biol Reprod. 91:60. PubMed Caruso R, et al. 2009. Gastroenterology. 136:2270. PubMed Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed Tapia-Abellán A, et al. 2021. Sci Adv. 7:eabf4468. PubMed Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed Geng T, et al. 2021. J Infect Dis. 223:2186. PubMed Nair S, et al. 2021. JCI Insight. 6:. PubMed Mori Y, et al. 2014. Sci Rep. 4:6997. PubMed Muri J, et al. 2020. eLife. 9:e53627.. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed Liu Y, et al. 2022. iScience. 25:105121. PubMed Guo D, et al. 2022. Bone Res. 10:45. PubMed Matsumoto A, et al. 2021. iScience. 24:102839. PubMed von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed Seki N, et al. 2022. iScience. 25:104838. PubMed Zhu Q, et al. 2022. Atherosclerosis. 357:41. PubMed Antsiferova M, et al. 2017. EMBO Mol Med. 9(1):27-45. PubMed Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed Qian Z, et al. 2022. Biomedicines. 10:. PubMed Bian Z, et al. 2012. J Immunol. 188:844. PubMed Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed Knox T, et al. 2019. Sci Rep. 9:6136. PubMed Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed Khoury MK, et al. 2021. JVS Vasc Sci. 1:123. PubMed Chakraborty K, et al. 2017. Nat Commun. 8:13944. PubMed Yi-Wei Lin, Emmanuel Montassier, Dan Knights 2016. Sci Rep. 6:38599. PubMed Liu Y, et al. 2022. Exp Anim. :. PubMed Lee HN, et al. 2022. JCI Insight. 7:. PubMed Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed Wang Y, et al. 2021. Nature. 599:308. PubMed Zhang B, et al. 2021. Nature. 599:471. PubMed Rahman H, et al. 2020. J Invest Dermatol. . PubMed Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed Ghosh M, et al. 2021. Cancer Cell. 39(4):494-508.e5. PubMed
RRID: AB_893491 (BioLegend Cat. No. 123119) AB_893479 (BioLegend Cat. No. 123120)
Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733
UniProt: View information about F4/80 on UniProt.org
Clone: BM8
Regulatory Status: RUO
Other Names: EMR1, Ly71
Isotype: Rat IgG2a, κ