Biolegend, 123130, Alexa Fluor® 700 anti-mouse F4/80 Antibody, 100μg

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine macrophages
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.
Application References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed Du Y, et al. 2022. FASEB J. 36:e22248. PubMed Kang J, et al. 2023. JCI Insight. 8: . PubMed Domingues CS, et al. 2020. Front Immunol. 11:566476. PubMed Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed Casadesús AV, et al. 2020. Oncoimmunology. 9:1770565. PubMed Redelinghuys P, et al. 2016. Ann Rheum Dis. 75: 1386 - 1391. PubMed Markey K, et al. 2014. J Immunol. 192:5426. PubMed Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed Wei W, et al. 2022. mSystems. 7:e0046922. PubMed Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed Dholakia J, et al. 2022. Gynecol Oncol. 164:170. PubMed Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed Gangoso E, et al. 2021. Cell. 184:2454. PubMed
RRID: AB_2277848 (BioLegend Cat. No. 123129) AB_2293450 (BioLegend Cat. No. 123130)
Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733
UniProt: View information about F4/80 on UniProt.org
Clone: BM8
Regulatory Status: RUO
Other Names: EMR1, Ly71
Isotype: Rat IgG2a, κ