Biolegend, 123417, APC/Cyanine7 anti-mouse CD21/CD35 (CR2/CR1) Antibody, 25μg

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD35/CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Tan J, et al. 2021. iScience. 24(8):102835. PubMed Borbet TC, et al. 2023. iScience. 26:106810. PubMed Worth AN, et al. 2022. Cell Rep. 39:110899. PubMed Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed Slobodin B, et al. 2020. Molecular Cell. 78(3):434-444.e5.. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Prados A, et al. 2021. Nat Immunol. 22:510. PubMed Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed Kwok T, et al. 2022. Front Aging. 3:838943. PubMed Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_1953274 (BioLegend Cat. No. 123417) AB_1953275 (BioLegend Cat. No. 123418)
Structure: 145 kD/190 kD type I transmembrane glycoprotein
Distribution: B cells, follicular dendritic cells
Function: Signal transduction
Ligand/Receptor: Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type: B cells, Dendritic cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
Gene ID: 1290212946
UniProt: View information about CD21/CD35 on UniProt.org
Clone: 7E9
Regulatory Status: RUO
Other Names: CR2/CR1
Isotype: Rat IgG2a, κ