Biolegend, 123614, PerCP/Cyanine5.5 anti-mouse CD104 Antibody, 100μg

CD104 is a 205 kD type I transmembrane glycoprotein, also known as integrin β4 chain or β4 integrin, that associates with integrin α6 (CD49f) to form the α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells, and a subset of pre-T cells. The CD49f/CD104 heterodimer is an adhesion receptor for laminins (especially laminin 5) and keratin filaments, and is involved in the regulation of hemidesmosome formation and cell proliferation and activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Tumor-associated antigen TSP-180 from BALB/c lung carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported (for the relevant formats) applications include: immunohistochemical staining1.
Application References(PubMed link indicates BioLegend citation): Tennenbaum T, et al. 1993. Cancer Res. 53:4803. (IHC)
Product Citations: Shi X, et al. 2022. Clin Transl Med. 12:e711. PubMed
RRID: AB_2734183 (BioLegend Cat. No. 123613) AB_2734184 (BioLegend Cat. No. 123614)
Structure: Integrin β chain, 205 kD, forms heterodimer with integrin α6 (CD49f)
Distribution: Epithelial cells, endothelial cells, some tumor cells, pre-T cell subset
Function: Adhesion
Ligand/Receptor: Laminins, keratin filaments
Cell Type: Embryonic Stem Cells, Endothelial cells, Epithelial cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kennel SJ, et al. 1992. J. Cell Sciences 101:1992.
Gene ID: 192897
UniProt: View information about CD104 on UniProt.org
Clone: 346-11A
Regulatory Status: RUO
Other Names: Integrin β4, β4 integrin, TSP-1180, ITGB4
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.