Biolegend, 126505, PE anti-mouse CD183 (CXCR3) Antibody, 25μg

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca 2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse N-terminus of CXCR3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Application References(PubMed link indicates BioLegend citation): Krug A, et al. 2002. J. Immunol. 169:6079. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed Barbi J, et al. 2009. FASEB J. 23:3990. PubMed Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations: Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed Cortés JR, et al. 2022. Cell Rep. 39:110695. PubMed Valencia–Hernandez AM, et al. 2020. Cell Host & Microbe. 27(6):950-962. PubMed Kim CJ, et al. 2018. Immunity. 49:1034. PubMed Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed Pein M, et al. 2020. Nat Commun. 11:1494. PubMed Yuan B, et al. 2021. J Immunother Cancer. 9:. PubMed Pais Ferreira D, et al. 2020. Immunity. 53(5):985-1000.e11. PubMed Sebastian M, et al. 2016. J Immunol. 196: 144 - 155. PubMed Guo Y, et al. 2020. Cell Host & Microbe. 28(1):104-116.e4.. PubMed Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed Barbi J, et al. 2009. FASEB J. 3.729166667. PubMed Engel I, et al. 2016. Nat Immunol. 10.1038/ni.3437. PubMed Wang Q, et al. 2013. Genes Dev. 27:1568. PubMed Kimpfler S, et al. 2009. J Immunol. 183:6330. PubMed Li H, et al. 2021. Protein Cell. Online ahead of print. PubMed Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Oghumu S, et al. 2013. J Immunol. 190:2229. PubMed McAleer J, et al. 2011. Int Immunol. 1.047916667. PubMed Fend L, et al. 2014. Cancer Immunol Res. 2:1163. PubMed Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
RRID: AB_1027656 (BioLegend Cat. No. 126505) AB_1027650 (BioLegend Cat. No. 126506)
Distribution: T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors
Function: Essential in T cell recruitment to sites of inflammation
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID: 12766
UniProt: View information about CD183 on UniProt.org
Clone: CXCR3-173
Regulatory Status: RUO
Other Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.