Biolegend, 126522, Brilliant Violet 421™ anti-mouse CD183 (CXCR3) Antibody, 500microl

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca 2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.
500microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse N-terminus of CXCR3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Application References(PubMed link indicates BioLegend citation): Krug A, et al. 2002. J. Immunol. 169:6079. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed Barbi J, et al. 2009. FASEB J. 23:3990. PubMed Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations: Wang X, et al. 2019. Cell Res. 29:787. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed Heindl S, et al. 2021. J Exp Med. 218:. PubMed Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed Jong RM, et al. 2022. J Immunol. 208:407. PubMed Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed Woodworth JS, et al. 2021. Nat Commun. 12:6658. PubMed Carpio V, et al. 2015. PLoS One. 10: 0144654. PubMed Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Todorov H, et al. 2022. iScience. 25:104927. PubMed Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed Sheikh AA, et al. 2019. Cell Rep. 28:1758. PubMed
RRID: AB_10900974 (BioLegend Cat. No. 126521) AB_2563100 (BioLegend Cat. No. 126529) AB_2562205 (BioLegend Cat. No. 126522)
Distribution: T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors
Function: Essential in T cell recruitment to sites of inflammation
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID: 12766
UniProt: View information about CD183 on UniProt.org
Clone: CXCR3-173
Regulatory Status: RUO
Other Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype: Armenian Hamster IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.