Biolegend, 126619, APC/Cyanine7 anti-mouse CD8b Antibody, 25μg

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
Application References(PubMed link indicates BioLegend citation): McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations: Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Francian A, et al. 2021. J Drug Target. 29:754. PubMed Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Chan WF, et al. 2022. Nat Commun. 13:5582. PubMed Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed Pavelko K, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01532. PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Wang M, et al. 2021. iScience. 24(7):102766. PubMed Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
RRID: AB_2563950 (BioLegend Cat. No. 126619) AB_2563951 (BioLegend Cat. No. 126620)
Structure: Ig superfamily, CD8β chain, 34 kD
Distribution: Majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand/Receptor: MHC class I
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
Gene ID: 12526
UniProt: View information about CD8beta on UniProt.org
Clone: YTS156.7.7
Regulatory Status: RUO
Other Names: Lyt-3, Ly-3
Isotype: Rat IgG2b, κ