Biolegend, 127105, Alexa Fluor® 647 anti-mouse CD317 (BST2, PDCA-1) Antibody, 25μg

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 was recently identified as an IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse plasmacytoid dendritic cells (DCs)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260.
Product Citations: Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed Hirata SI, et al. 2020. Allergy. 75:1939. PubMed Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
RRID: AB_2228222 (BioLegend Cat. No. 127105) AB_2067120 (BioLegend Cat. No. 127106)
Structure: Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution: Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
Function: Recently identified as antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type: Dendritic cells, B cells
Biology Area: Immunology, Innate Immunity, Costimulatory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Douglas JL, et al. 2009. J Virol. 83:7931.2. Cao W, et al. 2009. J. Exp. Med. 206:1603.3. Neil SJ, et al. 2008. Nature 451:425.
Gene ID: 69550
UniProt: View information about CD317 on UniProt.org
Clone: 129C1
Regulatory Status: RUO
Other Names: BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype: Rat IgG2b, κ