Biolegend, 127220, FITC anti-mouse CD73 Antibody, 100μg

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with mouse CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations: Volkova MV, et al. 2023. Int J Mol Sci. 24:. PubMed Xia KS, et al. 2023. Bioact Mater. 21:69. PubMed Fu H, et al. 2023. Regen Ther. 22:7. PubMed Tunali G, et al. 2023. J Clin Invest. :. PubMed Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Huang Z, et al. 2020. Mol Med Rep. 1.979166667. PubMed
RRID: AB_2716075 (BioLegend Cat. No. 127219) AB_2716076 (BioLegend Cat. No. 127220)
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor: Nucleoside 5' monophosphate
Cell Type: B cells, Mesenchymal Stem Cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.
Regulation: Expression is regulated by the development and maturation of leukocytes
Gene ID: 23959
UniProt: View information about CD73 on UniProt.org
Clone: TY/11.8
Regulatory Status: RUO
Other Names: Ecto-5'-nucleotidase
Isotype: Rat IgG1, κ